Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Ultra Violet 496, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue / Red / Violet / Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. It has tyrosine phosphatase activity and plays an essential role in lymphocyte activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤0.5 µg/test)
  • Defined as the amount of antibody staining a sample in 100 µL volume
  • Cell number: 10⁵–10⁸ cells/test (empirically determined)
  • Optimize titration for best performance

Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm and is designed for cytometers with a 355 nm UV laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42) or
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize nonspecific interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49) or
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Fluorophore Properties:

• Excitation: 350 nm
• Emission: 496 nm
• Laser: Ultraviolet

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (Leukocyte Common Antigen, LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). It is absent in non-hematopoietic tissues and cell lines.
CD45 regulates lymphocyte development, antigen receptor signaling, and cytokine receptor pathways by modulating Src-family and JAK kinases.
Isoforms (e.g., CD45RA) result from alternative splicing and vary by lymphoid cell subtype and physiological state. CD45 influences integrin-mediated signaling, apoptosis, and immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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