Thermo Fisher Scientific RTRAF Monoclonal Antibody (UMAB175), UltraMAB
Human RTRAF 단백질을 타깃으로 하는 UMAB175 클론 단일클론 항체. Western blot, IHC, ICC, ChIP 등 다양한 응용 가능. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공. 연구용으로만 사용.
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Thermo Fisher Scientific RTRAF Monoclonal Antibody (UMAB175), UltraMAB
Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | Assay-dependent | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 | |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 | |
| ChIP assay (ChIP) | Assay-dependent | |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB175 |
| Immunogen | Full-length human recombinant protein of C14orf166 (RTRAF) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
RTRAF (C14orf166) is an RNA-binding protein involved in modulation of mRNA transcription by Polymerase II (PubMed:16950395). It is a component of the tRNA-splicing ligase complex and is required for tRNA ligation (PubMed:24870230). May also play a role in RNA transport (PubMed:24608264).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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