Thermo Fisher Scientific H4R3me2a Polyclonal Antibody
H4R3me2a 다이메틸화 히스톤 H4(Arg3) 인식 폴리클로날 항체로, WB, IHC, ICC, IP, ChIP 등 다양한 응용에 적합합니다. Rabbit IgG로 제작되었으며, 친화 크로마토그래피로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | – |
| Immunoprecipitation (IP) | 1:50–1:200 | – |
| ChIP assay (ChIP) | 1:50–1:200 | – |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic methylated peptide derived from human Di-Methyl-Histone H4 around the methylation site of Arg3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2815927 |
Product Specific Information
This antibody detects endogenous levels of Di-Methyl-Histone H4 only when methylated at Arg3.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped to form nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes, facilitating chromatin compaction into higher-order structures. The gene encoding histone H4 is intronless, lacks a polyA tail, and contains a palindromic termination element. It is located within the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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