Thermo Fisher Scientific TEF1 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human TEF1 protein. Validated for WB, IHC, ICC/IF, and ChIP applications. Supplied as liquid form (2.16 mg/mL) and stored at -20°C. Suitable for detecting nuclear TEF1 in multiple tissues and cell lines.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ChIP assay (ChIP) | 1:20–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 200–300 of human TEAD1 (NP_068780.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.16 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913785 |
Product Specific Information
- Positive test controls: 293T, HeLa, Mouse skeletal muscle, Mouse heart, Rat heart
- Subcellular localization: Nucleus
Immunogen sequence:
APSVPAWQGR SIGTTKLRLV EFSAFLEQQR DPDSYNKHLF VHIGHANHSY SDPLLESVDI RQIYDKFPEK KGGLKELFGK GPQNAFFLVK FWADLNCNIQ D
Target Information
TEF1 (Transcriptional enhancer factor 1) is a nuclear protein belonging to the TEA/ATTS domain family, expressed in numerous cell types and involved in regulating gene expression.
TEF family proteins have a conserved DNA-binding domain. TEF-1 binds to GT-IIC, SphI/II, and M-CAT sequences, and also to the proximal regulatory element (PRE) of transforming growth factor-alpha, which is overexpressed in many cancers.
TEF-1 represses transcription in placental cells and is phosphorylated by PKC isozymes both in vivo and in vitro, reducing its DNA-binding capability. This suggests a potential role in PKC inhibition and tumorigenesis. Dimerization of TEF-1 may be essential for its transcriptional regulatory function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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