Thermo Fisher Scientific H3K27ac Polyclonal Antibody
H3K27ac에 특이적인 Rabbit Polyclonal 항체로, Western blot, ELISA, ChIP, Dot blot에 사용 가능. Human, Mouse, Rat 시료 반응성. 액상 형태로 1.67 mg/mL 농도, -20°C 보관. 크로마틴 구조 및 전사 조절 연구에 적합.
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- PA5116936
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution / Usage |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg of chromatin |
| Dot blot (DB) | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic acetylated peptide around K27 of human Histone H3 (NP_0035201) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.67 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901566 |
Product Specific Information
- Positive Samples: HeLa, NIH/3T3
- Immunogen sequence: ARKSA
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to alter chromatin structure or enable binding of specific transcription factors. The N-terminal tail of histone H3 can undergo multiple post-translational modifications such as methylation, acetylation, and phosphorylation, which influence cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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