Thermo Fisher Scientific SCAMP2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-734 detects secretory carrier membrane protein 2 (SCAMP2) from mouse testes, AtT20 cell extract, and Chinese hamster ovary (CHO) cell extract.
This antibody has been successfully used in Western blot procedures, detecting an approximately 38 kDa SCAMP2 protein from CHO cell extract.

The immunizing peptide corresponds to amino acid residues 1–17 from human SCAMP2, a sequence completely conserved between human and mouse.
The immunizing peptide (Cat. # PEP-151) is available for use in neutralization and control experiments.

Target Information

Secretory carrier membrane proteins (SCAMPs) are components of post-Golgi membranes involved in membrane trafficking.
In fibroblasts, SCAMPs are concentrated in compartments related to receptor recycling and endocytosis.
In neurons, SCAMPs are associated with synaptic vesicles, secretion granules, and transporter vesicles.

SCAMPs contain four central transmembrane regions and a cytoplasmic tail.
Of the five known SCAMPs, SCAMPs 1–3 have NPF repeats in their N-terminal regions, which interact with EH domain proteins involved in vesicle budding from the plasma membrane or Golgi complex.
SCAMPs 4 and 5 lack these N-terminal NPF repeats. SCAMPs 1–4 are ubiquitously coexpressed, while SCAMP5 is brain-specific and expressed later in development, coinciding with synapse maturation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.