Thermo Fisher Scientific HDAC2 Recombinant Rabbit Monoclonal Antibody (SY29-02)
HDAC2 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 감도를 제공. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. HEK293 발현 시스템 기반 생산으로 로트 간 일관성 확보. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:50,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY29-02 |
| Immunogen | Synthetic peptide within Human HDAC2 (aa 439–488) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809394 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
HDAC2 belongs to the histone deacetylase family, which acts via large multiprotein complexes to deacetylate lysine residues on core histones (H2A, H2B, H3, and H4).
HDAC2 forms transcriptional repressor complexes with proteins such as YY1, playing a key role in transcriptional regulation, cell cycle progression, and development.
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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