Thermo Fisher Scientific Phospho-NFkB p65 (Thr435) Polyclonal Antibody
NF-κB p65 (Thr435) 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. Affinity chromatography 정제, 1 mg/mL 농도, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of threonine 435 (E-G-T(p)-L-S) derived from Human NF-kappa-B-p65 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554332 |
Product Specific Information
- Suggested positive control for Western blot: HeLa cells
- Suggested positive control for IHC: human breast carcinoma
- Suggested positive control for ICC/IF: HeLa cells
Target Information
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene located on chromosome 11 in humans.
Also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A), it belongs to the Rel family of proteins and forms one of the two subunits of NF-kB, which heterodimerizes with p50 or p52.
Upon TNF-alpha binding to its receptor, IKK is phosphorylated, which phosphorylates IκB, leading to its proteasomal degradation. NF-kB p65 plays a key role in transcription of the immunoglobulin kappa gene in mature B-lymphoid cells.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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