Thermo Fisher Scientific Phospho-AKT1 (Ser473) Monoclonal Antibody (14-6)
Phospho-AKT1 (Ser473) 단일클론 항체로 인간 및 마우스 시료에 반응하며, Western blot, IHC, ICC 등 다양한 응용에 적합. Protein A 정제, 0.5 mg/mL 농도의 액상 형태로 제공. 세포 생존 및 신호전달 연구용으로 이상적.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 48 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| in situ PLA (PLA) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Chicken, Dog, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14-6 |
| Immunogen | Chemically synthesized phosphopeptide derived from human Akt region containing serine 473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | Dulbecco’s PBS (pH 7.3) with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533699 |
Product Specific Information
- Contains enough material for 10 mini-blots.
- Immunogenic sequence conserved among human, mouse, and rat in Akt1, Akt2, and Akt3 isoforms.
- Recommended positive controls: NIH-3T3 cells stimulated with PDGF, and 3T3-L1 adipocytes stimulated with OSM and IFN-gamma.
- Other species (100% homologous) not tested.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival by inhibiting apoptosis and promoting cell cycle progression.
Activated by insulin, PI3K, IGF1, and other growth factors, Akt phosphorylates various targets, including forkhead transcription factors and caspase-9.
The AKT pathway is a major focus in cancer drug discovery due to its role in cell survival and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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