Thermo Fisher Scientific TLR2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.5–2 µg/mL
• Publications: [References not provided]

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:10–1:500
• Publications: [References not provided]

Product Specifications

Product Specific Information

Suggested positive control: antigen standard for TLR2 (transient overexpression lysate), Ramos, RAW, or mouse TLR2 transfected cell lysate.

Target Information

TLR2 is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate kinases and transcription factors including PKC alpha/beta and NF-kappa-B.
TLR2 forms heterodimers with TLR1 or TLR6 to recognize bacterial and mycoplasma lipoproteins. It is highly conserved from Drosophila to humans and shows distinct expression patterns.
TLR2 is most abundantly expressed in peripheral blood leukocytes and mediates host responses to Gram-positive bacteria and yeast via NF-kappaB activation. It recognizes pathogen-associated molecular patterns (PAMPs) and triggers cytokine production for effective immunity.
TLR2 is expressed in human tonsils, microglia, lymph nodes, appendices, and activated B-cells in germinal centers. CD14+ monocytes show the highest TLR2 levels, followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. Expression is modulated by immune response modifiers: LPS, GM-CSF, IL-1, and IL-10 upregulate TLR2, while IL-4, IFN-gamma, and TNF downregulate it in monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.