Thermo Fisher Scientific ErbB 4 Polyclonal Antibody, FITC
ErbB4 단백질을 인식하는 FITC 결합 폴리클로날 항체로, Western blot, IHC, ELISA, IP 등 다양한 분석에 사용 가능. 인간, 마우스, 랫트에 반응하며 고순도 친화 크로마토그래피 정제. 형광 특성 498/517 nm, 연구용 전용 제품.
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Thermo Fisher Scientific ErbB 4 Polyclonal Antibody, FITC
Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 1225–1275 on human Receptor tyrosine-protein kinase erbB-4 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine kinase subfamily that includes EGFR, ERBB2, and ERBB3.
ERBB4 binds and is activated by neuregulins and other factors, inducing a variety of cellular responses including mitogenesis and differentiation.
It mediates tyrosine phosphorylation and plays a key role in signal transduction, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, apoptosis, and differentiation.
ErbB4 is predominantly expressed in several breast carcinoma cell lines and in normal skeletal muscle, heart, pituitary, brain, and cerebellum.
High levels are found in T47-D, MDA-MB-453, BT-474, and H3396, while intermediate levels are seen in MCF-7, MDA-MB-330, and MDA-MB-361.
Expression is low or absent in MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3.
Elevated ErbB4 levels in certain tumor cell lines suggest its involvement in malignancy.
주의사항
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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