Thermo Fisher Scientific STIM1 (extracellular) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

5-10 µg/1x10^6 cells

-

Immunoprecipitation (IP)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to amino acid residues 56-69 of human STIM1 (STIM1 expressed on the plasma membrane: Extracellular, N-terminus, STIM1 expressed on the ER: Luminal, N-terminus) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:STIM1,uniProtId:Q13586-1,ncbiNodeId:9606,antigenRange:57-69,antigenLength:685,antigenImageFileName:PA5-111756_STIM1_Q13586-1_Rabbit.svg,antigenImageFileNamePDP:PA5-111756_STIM1_Q13586-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.85 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2857166

Product Specific Information

Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Peptide sequence is: CHSEDEKLSF EAVR

Homology: Rat, mouse, bovine, canis amino acid residues are identical.

Target Information

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.