Thermo Fisher Scientific STIM1 (extracellular) Polyclonal Antibody
STIM1 단백질의 세포외 영역을 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. Ca²⁺ 신호 전달 연구 및 STIM1-Orai1 상호작용 분석에 유용. 연구용으로만 사용 가능.
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Thermo Fisher Scientific STIM1 (extracellular) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 5–10 µg/1×10⁶ cells |
| Immunoprecipitation (IP) | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide corresponding to amino acid residues 56–69 of human STIM1 (Extracellular, N-terminus) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.85 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2857166 |
Product Specific Information
- Reconstitute antibody with 50 µL of double distilled water (DDW).
- Store reconstituted solution at 4°C for up to 1 week. For longer storage, aliquot and keep at -20°C.
- Avoid repeated freeze/thaw cycles.
- Centrifuge antibody preparations before use (10,000 × g for 5 min).
- Peptide sequence: CHSEDEKLSF EAVR
- Homology: Rat, mouse, bovine, canis amino acid residues are identical.
Target Information
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Upon decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. STIM1 also associates with growing ends of microtubules and contributes to endoplasmic reticulum tubule extension.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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