Thermo Fisher Scientific RTF1 Polyclonal Antibody
Drosophila Rtf1 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot, ICC/IF, IP, ChIP 등 다양한 응용에 적합. Protein G로 정제된 고순도 항체로, -20°C 이하에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Immunoprecipitation (IP) | 12 µg |
| ChIP assay (ChIP) | 1–5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Drosophila Rtf1 (Rtf1, Paf1/RNA polymerase II complex component, homolog) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.8 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.05% ProClin 300, 0.05% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2576476 |
Target Information
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65, and gammaPAK p62, exhibit high sequence homology with the S. cerevisiae kinase Ste20, which is involved in pheromone signaling.
The alpha, beta, and gammaPAK isoforms specifically complex with Rac1 and Cdc42 in their active GTP-bound states, inhibiting their intrinsic GTPase activity and leading to autophosphorylation.
There are eight autophosphorylation sites on gammaPAK, including Ser19, Ser141, and Thr402; phosphorylation of Ser141 and Thr402 correlates with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms dissociate from the complex to act on downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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