Thermo Fisher Scientific RTF1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:50

-

Immunoprecipitation (IP)

12 µg

-

ChIP assay (ChIP)

1-5 µg

-

Product Specifications

Species Reactivity

Fruit fly

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant drosophila Rtf1 (Rtf1, Paf1/RNA polymerase II complex component, homolog) protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.8 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.05% ProClin 300, 0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576476

Target Information

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.