Thermo Fisher Scientific IL-4 Monoclonal Antibody (E10023)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

ELISA (ELISA)

0.005-20 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

E10023

Immunogen

Recombinant human IL-4 purified from Pichia pastoris

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 10% trehalose

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_417051

Product Specific Information

M3IL4I targets IL-4 in ELISA and Western blot applications and shows reactivity with Human samples.

The M3IL4I immunogen is recombinant human IL-4 purified from Pichia pastoris.

M3IL4I detects IL-4 which has a predicted molecular weight of approximately 15 kDa.

The M3IL4I IL4 antibody (clone E10023) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M2IL4BI (biotinylated conjugate of clone E10022). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody M3IL4I (clone E10022) and biotinylated antibody M2IL4BI (clone E10023) have successfully been used in combination with recombinant IL4 protein SIL4 in ELISA applications.

Endotoxin levels are less than or equal to 10 µg/mL.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.