Thermo Fisher Scientific IL-1 alpha Monoclonal Antibody (ILA8-H12), Biotin
인간 IL-1 alpha를 검출하는 비오틴 결합 단일클론 항체로 ELISA에 최적화되어 있습니다. 마우스 IgG1, kappa 아이소타입이며 Protein G로 정제되었습니다. -20°C 보관, 인간 시료 반응성, 재조합 IL-1 alpha를 면역원으로 사용합니다.
- 카탈로그번호
- M420AB
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific IL-1 alpha Monoclonal Antibody (ILA8-H12), Biotin
Applications
- ELISA (Assay-dependent)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ILA8-H12 |
| Immunogen | Recombinant human IL-1 alpha |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage buffer | PBS with 4% BSA |
| Contains | Proprietary preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_223564 |
Additional Formats
Product Specific Information
M420AB targets IL-1 alpha in ELISA applications and shows reactivity with Human samples.
The immunogen is recombinant human IL-1 alpha.
M420AB detects IL-1 alpha (~18 kDa predicted molecular weight).
Biotinylated antibody M420AB (clone ILA8-H12) has been successfully paired as the detection antibody in a sandwich ELISA with capture antibody M700A (clone 2G1).
Typical dilutions for sandwich ELISA include:
- Coating: 1 µg/mL
- Detection: 0.125–0.25 µg/mL
Biotinylated antibody M420AB (clone ILA8-H12) and antibody M421A (clone ILA9-H18) have also been used in combination with recombinant IL1 alpha protein SIL1A in ELISA applications.
Target Information
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells.
It is encoded by the IL1A gene located on chromosome 2q13 and signals through IL-1RI and IL-1RII receptors, shared with IL-1 beta.
IL-1 alpha plays a crucial role in innate host defense by inducing production of other proinflammatory cytokines and initiating acute-phase responses.
Its activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), which blocks receptor binding.
IL-1 alpha is pleiotropic, involved in immune responses, inflammation, and hematopoiesis.
Produced as a proprotein, it is processed and released upon cell injury, inducing apoptosis.
The IL1A gene forms part of a cytokine gene cluster on chromosome 2.
IL-1 alpha regulates NF-kappaB and MAPK activity, stimulates IL6 and COX-2 expression, and enhances nitric oxide production.
Elevated IL-1 alpha levels are linked to chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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