Thermo Fisher Scientific NKX2.2 Recombinant Mouse Monoclonal Antibody (rNX2, 294)
Recombinant Mouse Monoclonal Antibody targeting NKX2.2, suitable for IHC(P) and peptide array applications. Reacts with chicken, human, mouse, and rat. Produced in HEK293 cells, purified via Protein A/G, and supplied in PBS with BSA and sodium azide.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rNX2, 294 |
| Immunogen | Recombinant full-length human NKX2.2 protein (UniProt ID: O95096-1, antigen range: 1–273) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The NKX2.2 protein contains a homeobox domain and may play a role in central nervous system morphogenesis.
This gene is located on chromosome 20 near NKX2-4, and both are duplicated on chromosome 14 as TITF1 and NKX2-8.
The encoded protein is likely a nuclear transcription factor.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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