Thermo Fisher Scientific DBF4 Recombinant Rabbit Monoclonal Antibody (JE52-50)
DBF4(ASK) 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot 및 유세포분석에 적합합니다. 높은 특이성과 재현성을 제공하며, 단백질 발현 및 세포주기 연구에 활용됩니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE52-50 |
| Immunogen | Recombinant protein within Human DBF4 (aa 1–107) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2890386 |
Target Information
Activator of S phase kinase (ASK, DBF4) is a regulatory subunit of the human Cdc7 kinase complex, playing a critical role in the G1/S cell cycle transition. Increased expression of ASK in late G1 contributes to elevated Cdc7 kinase activity at the G1/S boundary. ASK interacts with replication origins, suggesting Cdc7 may trigger S phase by activating replication initiation complexes. Human ASK levels fluctuate during the cell cycle, paralleling Cdc7 activity. The Cdc7-ASK kinase complex regulates DNA replication initiation by targeting MCM2 protein. Nuclear localization and chromatin binding of Cdc7 and ASK are cell cycle–dependent, increasing at late G1 phase. A functional link may exist between ASK and the tumor suppressor menin (mutated in MEN1), implicating ASK in cell proliferation control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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