Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), FITC, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• View 2 publications

Flow Cytometry (Flow)

• Tested Dilution: 0.25 µg/test
• View 14 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• APC
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• eFluor 450
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

This TNKUPJ monoclonal antibody recognizes mouse CD163, a 130 kDa surface receptor expressed by specific subsets of tissue macrophages such as splenic red pulp macrophages, Kupffer cells, and intestinal lamina propria macrophages. Mouse monocytes do not express CD163, and its expression is not strongly induced by M2 polarizing cytokines, making it a poor M2 macrophage marker.

The TNKUPJ antibody detects CD163 on fixed and permeabilized cells, allowing intracellular staining. While CD163 is stable to collagenase digestion, aggressive tissue dissociation may reduce surface expression; in such cases, intracellular detection is recommended.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; recommended ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize concentration for best results.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and a member of the scavenger receptor cysteine-rich superfamily. It serves as a receptor for the hemoglobin–haptoglobin complex, protecting tissues from oxidative damage and facilitating iron recycling.

CD163 is expressed on human monocytes and macrophages, particularly in late inflammation stages. It is upregulated by IL-10, IL-6, and dexamethasone, and shed from the cell surface upon stimulation with LPS or PMA. The receptor binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner and initiates intracellular signaling leading to IL-6 and CSF1 secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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