Thermo Fisher Scientific PDHA1 Recombinant Rabbit Monoclonal Antibody (5N5W8)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:5,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

Immunogen Sequence:
IPGLRVDGMD ILCVREATRF AAAYCRSGKG PILMELQTYR YHGHSMSDPG VSYRTREEIQ EVRSKSDPIM LLKDRMVNSN LASVEELKEI DVEVRKEIED

Target Information

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO₂, linking glycolysis to the tricarboxylic acid (TCA) cycle. The PDH complex consists of multiple copies of three enzymatic components:

• Pyruvate dehydrogenase (E1)
• Dihydrolipoamide acetyltransferase (E2)
• Lipoamide dehydrogenase (E3)

The E1 enzyme is a heterotetramer of two alpha and two beta subunits. The PDHA1 gene encodes the E1 alpha 1 subunit, which contains the E1 active site and is essential for PDH complex function. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.