Thermo Fisher Scientific PDHA1 Recombinant Rabbit Monoclonal Antibody (5N5W8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5N5W8

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 248-348 of human PDHA1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PDHA1,uniProtId:P08559-1,ncbiNodeId:9606,antigenRange:248-348,antigenLength:390,antigenImageFileName:MA5-55220_PDHA1_P08559-1_Rabbit.svg,antigenImageFileNamePDP:MA5-55220_PDHA1_P08559-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen Sequence: IPGLRVDGMD ILCVREATRF AAAYCRSGKG PILMELQTYR YHGHSMSDPG VSYRTREEIQ EVRSKSDPIM LLKDRMVNSN LASVEELKEI DVEVRKEIED

Target Information

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.