Thermo Fisher Scientific alpha-Tubulin Monoclonal Antibody (17H11.F10)
모든 생물종에서 알파 튜불린을 인식하는 모노클로날 항체로, 로딩 컨트롤에 적합합니다. WB, IHC, ICC, ELISA, IP 등 다양한 응용 가능. 높은 특이성과 재현성을 제공하며, Protein A로 정제된 액상 형태로 안정적 보관이 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.1 µg/mL |
| ELISA | 1:300,000 |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Chicken, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17H11.F10 |
| Immunogen | Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C-terminal end of human alpha tubulin protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This Loading Control antibody is directed against alpha tubulin. BLAST analysis suggests antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian, aquatic, parasitic, and algal sources based on 100% homology for the immunogen sequence. Cross-reactivity occurs with all isoforms of alpha tubulin, making this antibody an excellent loading control.
Target Information
Alpha Tubulin forms a heterodimer with beta tubulin subunits, each approximately 55 kDa. This heterodimer is the basic building block of microtubules, which are cylindrical filamentous structures found in nearly all eukaryotic cells.
Microtubules provide structural support, intracellular transport pathways, and play key roles in mitosis. Gamma tubulin is involved in microtubule nucleation. Multiple alpha and beta tubulin genes are highly conserved among species.
The gene encoding alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Expression is primarily found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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