Thermo Fisher Scientific BUB1 Monoclonal Antibody (14H5)
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인간 BUB1 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot, ICC/IF, ELISA, IP 등에 사용 가능. Protein A로 정제된 액상 항체이며, -20°C에서 보관. 연구용으로 스핀들 체크포인트 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14H5 |
| Immunogen | Recombinant protein corresponding to amino acid residues 281–419 of human BUB1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02 M potassium phosphate (pH 7.2), 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid; dilute only before immediate use.
- Directed against human BUB1 protein.
- Purified from tissue culture supernatant by chromatography.
- Reacts with BUB1 from human cells; no reactivity with Xenopus or Kangaroo Rat homologues.
- Reactivity to other species unknown.
Target Information
BUB1A is a kinase involved in spindle checkpoint function. It phosphorylates a member of the mitotic checkpoint complex, thereby activating the spindle checkpoint mechanism.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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