Thermo Fisher Scientific NR4A1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive control: HepG2
• Predicted reactivity: Cow (100%), Dog (100%), Guinea Pig (93%), Horse (100%), Mouse (92%), Rabbit (93%), Rat (93%)
• In Western Blots, dilute using a 5% skim milk/PBS buffer solution.
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung, and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.