Thermo Fisher Scientific StAR (Steroidogenic Acute Regulator) Monoclonal Antibody (STAR, 2154)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Steroidogenic acute regulatory protein (StAR) is crucial for cholesterol transport from the cytoplasmic outer membrane to the inner mitochondrial membrane. The 37 kDa precursor is cleaved to an active 28 kDa protein that facilitates cholesterol metabolism into pregnenolone. StAR is predominantly expressed in mitochondria of steroid-producing adrenal and gonadal tissues. Abnormalities in StAR gene expression are associated with autosomal Lipoid Congenital Adrenal Hyperplasia (LCAH), leading to defects in pregnenolone and cortisol synthesis. The exact mechanism of cholesterol binding to StAR remains under investigation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.