Thermo Fisher Scientific SRC3 Monoclonal Antibody (AX15.3)
SRC3(AIB1) 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, IP, ChIP 등 다양한 연구용 응용에 적합함. 인간 시료 반응성이 있으며, 정제된 액상 형태로 제공됨. -20°C 보관, PBS/BSA 버퍼 포함.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:100 | – |
| Immunoprecipitation (IP) | Assay-dependent | – |
| ChIP assay (ChIP) | Assay-dependent | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AX15.3 |
| Immunogen | GST fusion protein encoding amino acid residues 605–1294 of human AIB1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325446 |
Product Specific Information
MA1-845 detects amplified in breast cancer-1 (AIB1) in human cells.
It has been successfully used in Western blot, immunoprecipitation, ChIP, and immunohistochemistry procedures.
By Western blot, this antibody detects an ~155 kDa protein representing AIB1 from MCF-7 cell extract.
The MA1-845 immunizing peptide corresponds to amino acid residues 605–1294 of human AIB1.
Target Information
AIB1-AC3 recognizes AIB1, a member of the SRC-1 family of nuclear receptor coactivators with an apparent molecular weight of 160 kDa.
The AIB1 gene has been mapped to region 20q12 and is amplified in breast tumors and pancreatic cancer cell lines.
AIB1 interacts in a ligand-dependent manner with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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