Thermo Fisher Scientific Vinculin Polyclonal Antibody
Vinculin 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합합니다. Human, Mouse, Rat에서 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 부착 및 세포골격 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 94–348 of Human Vinculin (UniProt ID: P18206-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.67 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547162 |
Product Specific Information
- Recommended positive controls: NT2D1, Mouse brain, HeLa
- Predicted reactivity: Human (99%), Mouse (98%), Rat (98%), Pig (98%), Chicken (93%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. Vinculin interacts with talin, integrin, and α-actinin, forming a key linkage between the extracellular matrix and cytoplasmic microfilament system. Mutations in the VCL gene can result in cardiomyopathy (dilated 1W or familial hypertrophic 15).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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