Thermo Fisher Scientific Phospho-PRAS40 (Ser202) Polyclonal Antibody
PRAS40 단백질의 Ser202 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal 항체. Western blot과 IHC(Paraffin) 적용 가능. 인간, 마우스, 랫트 반응성. 액상 형태로 1 mg/mL 농도, -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AKT1S1 (Accession Q96B36), corresponding to amino acid residues around phosphorylated Ser202. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817114 |
Product Specific Information
Antibody detects endogenous levels of PRAS40 only when phosphorylated at Ser202.
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals.
AKT1S1 (PRAS40) is a proline-rich substrate of the kinase AKT1 and plays a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia.
It is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and acts as a negative regulator of autophagy.
Treatment with insulin-like growth factor-1 (IGF1) can induce phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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