Thermo Fisher Scientific Nitrotryptophan Monoclonal Antibody (2D12)
6-Nitrotryptophan을 특이적으로 인식하는 마우스 단클론 항체. Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능. 비결합형 액상 포맷으로 제공되며, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Flow Cytometry (Flow) | Assay-Dependent | |
| ELISA | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D12 |
| Immunogen | Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735289 |
Product Specific Information
A 1:1,000 dilution of MA5-27569 was sufficient for detection of 6-Nitrotryptophan in 1 µg of 6-Nitrotryptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 6-Nitrotryptophan modified proteins. Does not cross-react with 7-Ketocholesterol modified proteins.
Target Information
6-Nitrotryptophan (6-NO₂-Trp) is one of the most stable and major ONOO⁻-modified amino acids. Growing evidence suggests that nitrotryptophan may play a significant role in cellular regulation under nitrosative stress.
Reactive nitrogen species (RNS) are formed in various diseases such as atherosclerosis, inflammation, cancer, and neurodegenerative diseases.
Peroxynitrite (ONOO⁻), a potent biological oxidant formed via a rapid reaction between nitric oxide (NO) and superoxide (O₂⁻), can induce nitration of aromatic amino acids such as Tyr, Trp, Phe, and His, resulting in modulation of protein functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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