Thermo Fisher Scientific Nitrotryptophan Monoclonal Antibody (4F8)
6-Nitrotryptophan 인식 단일클론 항체로 다양한 nitrosative stress 관련 연구에 활용 가능. Western blot, ICC/IF, ELISA 등에서 사용 가능. 1 mg/mL 액상 형태로 제공되며, -20°C 보관. 비결합형(mouse IgG2b) 항체.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50 | - |
| ELISA | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F8 |
| Immunogen | Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735290 |
Product Specific Information
A 1:1,000 dilution of MA5-27562 was sufficient for detection of 6-Nitrotryptophan in 1 µg of 6-Nitrotryptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 6-Nitrotryptophan modified proteins. Does not cross-react with 7-Ketocholesterol modified proteins.
Target Information
6-Nitrotryptophan (6-NO2-Trp) is one of the most stable and major ONOO⁻-modified amino acids. Growing evidence suggests that nitrotryptophan may play a significant role in cellular regulation under nitrosative stress.
Reactive nitrogen species (RNS) are formed in various diseases such as atherosclerosis, inflammation, cancer, and neurodegenerative diseases.
Peroxynitrite (ONOO⁻), a potent biological oxidant formed by the rapid reaction between nitric oxide (NO) and superoxide (O₂⁻), can induce nitration of aromatic amino acids such as Tyr, Trp, Phe, and His, resulting in modulation of protein function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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