Thermo Fisher Scientific 4-Hydroxynonenal Monoclonal Antibody (12F7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50

View 1 publication 1 publication

ELISA (ELISA)

1:1,000

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

12F7

Immunogen

Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH)

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735095

Product Specific Information

A 1:1,000 dilution of MA5-27570 was sufficient for detection of 4-Hydroxynonenal in 0.5 µg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

Target Information

4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, is an alpha, beta-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha, beta-unsaturated hydroxyalkenal formed in this process.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.