Thermo Fisher Scientific 4-Hydroxynonenal Monoclonal Antibody (12F7)
4-Hydroxynonenal(4-HNE) 변형 단백질을 특이적으로 인식하는 마우스 단일클론 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. 단백질 G로 정제된 액상 제품으로, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | 8 publications |
| Immunohistochemistry (IHC) | - | 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50 | 1 publication |
| ELISA | 1:1,000 | - |
| Immunoprecipitation (IP) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical, Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12F7 |
| Immunogen | Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735095 |
Available Conjugated Formats
Product Specific Information
A 1:1,000 dilution of MA5-27570 was sufficient for detection of 4-Hydroxynonenal in 0.5 µg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal (4-HNE, also known as 4-hydroxy-2-nonenal) is an α,β-unsaturated hydroxyalkenal produced by lipid peroxidation in cells. It is the primary α,β-unsaturated hydroxyalkenal formed in this process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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