Thermo Fisher Scientific Phospho-hnRNP D (Ser83) Polyclonal Antibody
Phospho-hnRNP D (Ser83) 폴리클로날 항체로 Western blot, IHC, ICC/IF에 적합. 인간 및 생쥐 반응성, Rabbit IgG 기반. 고순도 친화성 크로마토그래피 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of Serine 83 (N-S-S(p)-P-R) derived from Human hnRPD |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554204 |
Product Specific Information
- Suggested positive control for Western blot: JK cells
- Suggested positive control for IHC: Human lung carcinoma
- Suggested positive control for ICC/IF: HeLa cells
Target Information
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid-binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and influence pre-mRNA processing, mRNA metabolism, and transport.
While all hnRNPs are present in the nucleus, some shuttle between the nucleus and cytoplasm. The hnRNP proteins have distinct nucleic acid-binding properties. The encoded protein has two quasi-RRM domains that bind RNAs and localizes to both nucleus and cytoplasm. It is involved in mRNA stability regulation, and alternative splicing of this gene results in four transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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