Thermo Fisher Scientific PI3K p85 alpha Monoclonal Antibody (U5)
PI3K p85 alpha 단백질을 인식하는 Mouse Monoclonal Antibody (Clone U5). Western blot, IHC, ICC, IP 등 다양한 실험에 사용 가능. Bovine, Human, Mouse, Rat 등에서 반응. Protein A로 정제된 액상 형태로 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:1,000 | View 9 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
| Neutralization (Neu) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | U5 |
| Immunogen | Recombinant bovine p85 alpha subunit of PI-3 kinase, expressed in Baculovirus |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2163452 |
Product Specific Information
Mouse anti PI-3 Kinase p85 Subunit alpha antibody, clone U5 recognizes the bcr-homology domain of the p85 alpha subunit of bovine PI 3 kinase.
Target Information
Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases through its SH2 domain and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
PIK3 plays an important role in the metabolic actions of insulin, and mutations in this gene have been associated with insulin resistance. During insulin stimulation, it binds to IRS-1. Mutations can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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