Thermo Fisher Scientific Interferon gamma (IFNG) Monoclonal Antibody (IFNG/466)
인간 IFN-γ를 특이적으로 인식하는 단클론 항체로, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. 고순도 Protein A/G 정제, 액상 형태로 제공되며, -20°C에서 안정적 보관 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IFNG/466 |
| Immunogen | Purified recombinant human full-length IFNG protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Target Information
Interferon gamma (IFN-γ, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and natural killer cells in response to antigens and cytokines. It is a dimeric glycoprotein composed of two 146 amino acid subunits (~45 kDa functional homodimer). On SDS-PAGE, it appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Human IFN-γ exhibits species-specific activity and does not cross-react with mouse IFN-γ. Functions include:
- Antiviral and tumor antiproliferative activity
- Induction of MHC class I and II
- Macrophage activation
- Enhancement of immunoglobulin secretion by B lymphocytes
Activation occurs via binding to IFN-γ receptor I and II, triggering the JAK-STAT pathway. Human IFN-γ shares ~40% sequence homology with mouse IFN-γ. It is upregulated by IL2, FGF basic, EGF, and downregulated by vitamin D3 or DMN. Mutations in the IFN-γ gene are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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