Thermo Fisher Scientific PIGY Polyclonal Antibody
PIGY 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC에 사용 가능. Human과 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS buffer에 0.02% sodium azide 포함, 2–8°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 16 amino acid peptide near the center of human PIG-Y |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at –20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152560 |
Product Specific Information
Despite its predicted molecular weight, PIG-Y often migrates at 28–30 kDa in SDS-PAGE.
A suggested positive control is human spleen tissue lysate.
PA5-20652 can be used with blocking peptide PEP-0772.
Target Information
Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum.
The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2.
PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI.
The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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