Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.8 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin, eFluor 450, FITC
• Functional Grade
• NovaFluor Blue 510, 555, 585, 660-120S, 690, 725, 760, 800
• NovaFluor Red 700, 710, 725, 755
• NovaFluor UV 765
• NovaFluor Violet 690, 800
• NovaFluor Yellow 590, 610, 660, 690, 700, 730, 810
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The OKT8 monoclonal antibody recognizes human CD8a, a 32–34 kDa cell surface receptor expressed on thymocytes, a subset of mature T cells, and NK cells. CD8 binds to MHC class I molecules and associates with protein tyrosine kinase p56lck, contributing to T-cell development and activation.

Preliminary testing indicates that OKT8 does not compete with other anti-human CD8 clones (RPA-T8, HIT8a) for binding, suggesting distinct epitopes.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of normal human peripheral blood cells
  • Recommended use: 5 µL (0.8 µg)/test
  • A test = antibody amount for 100 µL final volume
  • Cell range: 10⁵–10⁸ cells/test

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Product # C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein in the immunoglobulin receptor family. It functions as a co-receptor with TCR for MHC class I molecules, playing a key role in T-cell activation and differentiation. CD8 is expressed mainly as a heterodimer (CD8αβ) on T cells and as a homodimer (CD8αα) on subsets of T cells, NK cells, monocytes, and dendritic cells. Upon MHC-I binding, CD8 recruits Lck kinase, initiating signaling that drives cytotoxic T lymphocyte (CTL) activation and pathogen or tumor cell clearance. Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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