Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Blue 690, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Blue 690

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test
Publications: N/A

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin, eFluor 450, FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The OKT8 monoclonal antibody reacts with the human CD8a molecule (~32–34 kDa), expressed as CD8αβ heterodimers or CD8αα homodimers on thymocytes, mature T cells, and NK cells. CD8a binds to MHC class I and associates with p56lck, playing a critical role in T-cell development and activation.

Preliminary testing shows OKT8 does not compete with clones RPA-T8 and HIT8a for binding, indicating distinct epitopes.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells (5 µL/test).
A test corresponds to staining in 100 µL volume with 10⁵–10⁸ cells.

Note: NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Product # C001T06F01) must be used for optimal staining.
Add 5 µL per sample before mixing with cells.

NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures, engineered for optimized fluorescence with minimal spillover.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

CD8 is a type I transmembrane glycoprotein functioning as a co-receptor for MHC class I molecules. It is expressed on T cells, NK cells, monocytes, and dendritic cells, forming CD8αβ heterodimers or CD8αα homodimers.
CD8 engagement with MHC-I/peptide complexes recruits Lck kinase, triggering cytokine production, motility, and cytotoxic T lymphocyte (CTL) activation.
CTL differentiation is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지