Thermo Fisher Scientific Phospho-AKT (Ser473) Monoclonal Antibody (17F6.B11), Atto 594
Phospho-AKT (Ser473) 단백질을 검출하는 Atto 594 형광 표지 단일클론 항체. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합. 인간, 생쥐, 랫드 등 반응성. Protein A 정제, 안정한 보관 조건 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | 1:20,000 |
| Immunoprecipitation (IP) | >1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17F6.B11 |
| Immunogen | Synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein. Produced by repeated immunizations. |
| Conjugate | Atto™ 594 |
| Excitation / Emission Max | 602 / 622 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if not clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human and mouse AKT protein phosphorylated at S473. BLAST analysis suggests cross-reactivity with AKT pS473 from human, mouse, rat, and chimpanzee (100% homology with immunizing sequence). Cross-reactivity with AKT2 and AKT3 has not been determined.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating multiple targets in various cell types, including cancer cells. It is activated by insulin, PI3K, IGF1, and other growth factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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