Thermo Fisher Scientific Phospho-BAD (Ser75) Polyclonal Antibody
Phospho-BAD (Ser75) 폴리클로날 항체로 인간, 생쥐, 랫트 반응성. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. Protein A로 정제된 고순도 항체이며 액상 형태로 제공. 세포 사멸 조절 관련 연구용으로 적합.
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Thermo Fisher Scientific Phospho-BAD (Ser75) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human BAD around the phosphorylation site of Ser75 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by BAD is a member of the BCL-2 family, known to regulate programmed cell death. BAD promotes apoptosis by forming heterodimers with BCL-xL and BCL-2, reversing their anti-apoptotic functions. Its proapoptotic activity is regulated through phosphorylation by kinases such as AKT and MAP kinase, as well as by calcineurin phosphatase. Alternative splicing produces two transcript variants encoding the same isoform.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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