Thermo Fisher Scientific Coilin Polyclonal Antibody
Rabbit polyclonal antibody against human Coilin protein for WB, IHC, and IP applications. Targets residues 526–576 of Coilin. Supplied as liquid, unconjugated form, 1 mg/mL concentration. Stored at 4°C, shipped on wet ice.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 526 and 576 of human Coilin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
Target Information
The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles that participate in post-transcriptional modification of small nuclear and small nucleolar RNAs.
The N-terminus of coilin directs self-oligomerization, while the C-terminus influences the number of nuclear bodies assembled per cell.
Differential methylation and phosphorylation of coilin likely affect its localization among nuclear bodies and the composition and assembly of Cajal bodies.
This gene has pseudogenes on chromosome 4 and chromosome 14.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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