Thermo Fisher Scientific Coilin Polyclonal Antibody
Human Coilin 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제된 액상 제품이며, 4°C 보관. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 526 and 576 of human Coilin (UniProt ID: P38432-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). These are nuclear suborganelles involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs.
The N-terminus of the coilin protein directs its self-oligomerization, while the C-terminus influences the number of nuclear bodies assembled per cell.
Differential methylation and phosphorylation of coilin likely influence its localization among nuclear bodies and the composition and assembly of Cajal bodies.
This gene has pseudogenes on chromosome 4 and chromosome 14.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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