Thermo Fisher Scientific CST2 Monoclonal Antibody (OTI5C2), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The cystatin superfamily comprises cysteine protease inhibitors.

• Type 1 (Cystatins A and B): Mainly intracellular
• Type 2 (Cystatins C, D, E/M, F, G, S, SN, SA): Extracellular
• Type 3 (Kininogens): Intravascular proteins

All true cystatins inhibit cysteine peptidases of the papain family (e.g., cathepsins) and some inhibit legumain family enzymes.
Cystatin SA, S, and SN are primarily found in saliva, and also expressed in tears, urine, and seminal fluid.
Cystatin C is expressed in brain, thymus, ovary, epididymis, and vas deferens.
Cystatin D protects against oral cavity proteases, while Cystatin E/M and F moderate cathepsin inhibition.
Fetuins, part of the cystatin superfamily, influence osteogenesis, bone resorption, and systemic inflammation response.
High and low molecular weight kininogens inhibit thrombin- and plasmin-induced thrombocyte aggregation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.