Thermo Fisher Scientific Phospho-SRC (Ser75) Polyclonal Antibody
SRC 단백질의 Ser75 인산화 상태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat 시료 반응성. PBS/glycerol buffer에 보관, -20°C에서 안정적 유지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SRC (Accession P12931), corresponding to amino acid residues around phosphorylated Ser75. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816266 |
Product Specific Information
This antibody detects endogenous levels of Src only when phosphorylated at Serine 75.
Target Information
SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. It regulates growth and differentiation of eukaryotic cells, associates with cytoplasmic regions of various cell surface receptors to facilitate signal transduction, and participates in cytoskeletal organization, cell cycle control, and apoptosis.
In humans, the SRC gene is located on chromosome 20. Its activity is regulated by phosphorylation at two tyrosine residues (Tyr416 and Tyr527), which have opposing effects: phosphorylation at Tyr416 activates the enzyme, while phosphorylation at Tyr527 inhibits it. Mutations in SRC may contribute to malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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