Thermo Fisher Scientific NOTCH3 Polyclonal Antibody
Mouse NOTCH3 단백질을 인식하는 Goat Polyclonal Antibody로, WB, IHC, Flow Cytometry, Neutralization 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 보존제 무첨가 상태로 -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (IHC) | 5–15 µg/mL |
| Flow Cytometry (Flow) | 2.5 µg per million cells |
| Neutralization (Neu) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Sf21-derived recombinant mouse Notch-3 (Ala40–Glu468) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607362 |
Product Specific Information
- In direct ELISAs, less than 10% cross-reactivity with recombinant human Notch-3 is observed.
- Less than 5% cross-reactivity with recombinant rat Notch-1 and Notch-2.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level: <0.10 EU per 1 µg of antibody (LAL method).
Target Information
NOTCH3 is the third discovered human homologue of the Drosophila melanogaster type I membrane protein notch. In Drosophila, notch interactions with ligands (delta, serrate) establish an intercellular signaling pathway essential for neural development.
Human homologues of these ligands have been identified, and NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell fate determination.
Mutations in NOTCH3 are known to cause cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: PA5-47120_NOTCH3_Q61982-1_Goat.svg, PA5-47120_NOTCH3_Q61982-1_Goat_PDP.jpeg)
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