Thermo Fisher Scientific APIP Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.

Target Information

The mammalian homologues of the key cell death gene CED-4 in C. elegans have been identified in human and mouse and designated Apaf-1 (apoptosis protease-activating factor 1). Apaf-1 binds to cytochrome c (Apaf-2) and Caspase-9 (Apaf-3), leading to Caspase-9 activation. Activated Caspase-9 subsequently cleaves and activates Caspase-3, a key protease responsible for the proteolytic cleavage of many proteins during apoptosis.

Recently, Cho et al. identified a new Apaf-1 interacting protein (APIP), also known as CG129 and MMRP19, which acts as a negative regulator of ischemic injury. APIP competes with the Caspase-9 binding site of Apaf-1. APIP is predicted to encode a 204 amino acid protein. An isoform, APIP2, encodes a 242 amino acid protein, differing in its N-terminus due to alternative splicing. APIP transcripts are ubiquitously expressed in most adult tissues, with high expression in skeletal muscle, heart, and kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.