Thermo Fisher Scientific Aurora B Polyclonal Antibody
Aurora B 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, ICC/IF, Flow cytometry, IP에 사용 가능. Human, Mouse, Rat 등 다양한 종에 반응하며 항원 친화 크로마토그래피로 정제됨. -20°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:50 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the amino terminus of human Aurora B/AIM1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 27 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10985872 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. The chromosomal passenger complex (CPC) also includes INCENP, survivin, and borealin, which regulate activity, localization, and stability of Aurora B. Two CPC forms exist during mitosis: one containing all four members and another consisting of INCENP and Aurora B. The full CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may modify histone H3. Aurora B is overexpressed in various tumors, including breast and colon cancers, and may interact with Aurora-A kinase through a feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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