Thermo Fisher Scientific PFKFB3 Recombinant Rabbit Monoclonal Antibody (JM43-43)
PFKFB3 단백질을 인식하는 Recombinant Rabbit Monoclonal Antibody (JM43-43). Western blot, IHC, ICC, Flow cytometry에 적합. 높은 특이성과 재현성을 가진 in vitro 생산 항체로, 인간, 생쥐, 랫트 시료에 반응. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM43-43 |
| Immunogen | Recombinant protein within Human PFKFB3 aa 260–520 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810043 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened for optimal performance.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
PFKFB3 encodes a bifunctional enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a key regulator of glycolysis.
This protein exhibits:
- 6-phosphofructo-2-kinase activity (synthesis of F2,6BP)
- Fructose-2,6-biphosphatase activity (degradation of F2,6BP)
PFKFB3 is essential for cell cycle progression and apoptosis prevention. It regulates cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and tumor cell survival.
Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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