Thermo Fisher Scientific B-Raf Monoclonal Antibody (CL4004)
인간 B-Raf 단백질을 인식하는 Mouse IgG2b 단일클론 항체로, WB 및 IHC(P)에서 검증됨. Protein A 정제, PBS/glycerol buffer에 보존. 암 관련 BRAF 신호전달 연구용으로 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CL4004 |
| Immunogen | Recombinant Protein corresponding to Human B-Raf (amino acids 299–447) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2848925 |
Product Specific Information
Immunogen sequence:
PIPQEEASLA ETALTSGSSP SAPASDSIGP QILTSPSPSK SIPIPQPFRP ADEDHRNQFG QRDRSSSAPN VHINTIEPVN IDDLIRDQGF RGDGGSTTGL SATPPASLPG SLTNVKALQK SPGPQRERKS SSSSEDRNRM KTLGRRDSS
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neurons. This protein regulates the MAP kinase/ERKs signaling pathway, affecting cell division, differentiation, and secretion. Mutations in B-Raf are associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and lung adenocarcinoma. A pseudogene located on chromosome X has been identified for B-Raf.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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