Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.015 µg)/test
• Publications: View 36 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 780
• Request custom conjugation

Product Specific Information

The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and expressed as a disulfide-linked dimer on the cell surface. It is rapidly upregulated upon activation and expressed on lymphocytes, monocytes, and platelets.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometric analysis of stimulated human peripheral blood cells
• Recommended Usage: 5 µL (0.015 µg) per test (in 100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. In normal peripheral blood, CD69 expression varies among cells and plays a role in lymphocyte signal transduction and proliferation. It may also transmit activation signals in NK cells and platelets. Alternative splicing results in multiple transcript variants.
Diseases associated with CD69 dysfunction include coccidioidomycosis and asthma.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지