Thermo Fisher Scientific CD62E (E-selectin) Polyclonal Antibody
CD62E (E-selectin) 폴리클로날 항체로 인간 시료에 반응하며 WB, ICC/IF, Flow Cytometry에 적합. 항원 친화 크로마토그래피로 정제된 고순도 항체. 염증 반응 관련 혈관 내피세포 연구에 활용 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within Human CD62E (aa 26–75) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% Glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802678 |
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. Leukocyte adhesion also occurs in capillaries and small venules throughout the body after the onset of inflammation. This immediate response involves a cascade of adhesion receptors. On the endothelial surface, members of the selectin and immunoglobulin superfamilies participate in this cascade.
Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the vascular endothelium. E-selectin (CD62E) is an endothelial cell-specific selectin expressed only after activation with proinflammatory cytokines. In vitro studies show that IL-1, TNFα, and bacterial components such as lipopolysaccharides induce E-selectin transcription via an NFκB-dependent signaling cascade. E-selectin is associated with blood vessel endothelium in various inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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