Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), PE-Cyanine5, eBioscience
마우스 CD31(PECAM-1)을 인식하는 단클론 항체로, PE-Cyanine5 형광 표지. 유세포 분석에 최적화되어 있으며, 0.5 µg/test로 사용 가능. 4°C 암소 보관, 광산화에 민감. 혈관 및 면역세포 연구용.
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- 15-0311-8x (2개 옵션)
- 판매단위
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), PE-Cyanine5, eBioscience
Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), PE-Cyanine5, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunocytochemistry (ICC/IF) | - | View 6 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 88 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 390 |
| Conjugate | PE-Cyanine5 |
| Excitation/Emission Max | 568 / 666 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848276 |
Available Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 711
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130–140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. CD38 has been reported to bind CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.5 µg/test (100 µL final volume). Optimize empirically based on cell number (10⁵–10⁸ cells/test).
Light Sensitivity: Tandem dye sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency.
Excitation: 488–561 nm
Emission: 667 nm
Laser: Blue, Green, Yellow-Green Laser
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor regulating T cell and B cell signaling via ITIM motifs that, upon phosphorylation, recruit protein-tyrosine phosphatases. It is expressed ubiquitously within the vascular compartment, mainly at junctions between adjacent cells. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions.
CD31 plays diverse roles in:
- Integrin-mediated adhesion
- Transendothelial migration
- Angiogenesis and apoptosis
- Negative regulation of immune signaling
- Autoimmunity and thrombosis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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