Thermo Fisher Scientific Rat Androgen Receptor, Ligand Binding Domain, GST Tag, His Tag Recombinant Protein

Thermo Fisher Scientific Rat Androgen Receptor, Ligand Binding Domain, GST Tag, His Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution

• Assay-dependent

Product Specifications

Product Specific Information

Androgen receptor ligand binding domain (AR-LBD) is a 64.42 kDa recombinant rat protein, expressed as a GST- and His-tagged protein in insect cells. The protein includes the hinge region and an LBD amino acid sequence identical to the human LBD, with differences in the hinge region at human amino acids 646, 649, 650, 655, 656, 657, and 660.

AR-LBD is expressed and purified in the absence of exogenous ligands, making it ideal for ligand-binding and coregulator recruitment studies.

Key Features:

• Flexible — Use in your in-house assay or as part of PolarScreen™ Competitor assays for a complete assay solution
• Ready to use — Active and tagged (GST/His)
• Convenient — Available in bulk sizes

Contact Thermo Fisher Scientific for bulk quantities or assistance with other nuclear receptor assay needs, including assay development and library screening.

Protein Form: Ligand Binding Domain, Recombinant

Target Information

The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors. It has three major functional domains: the N-terminal domain, DNA-binding domain, and the androgen-binding domain. AR acts as a ligand-activated transcription factor that binds testosterone (T) and dihydrotestosterone (DHT). Upon ligand binding, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and activates transcription of androgen-responsive genes.

The AR signaling pathway is crucial for the development and function of male reproductive organs such as the prostate and epididymis, and also plays roles in muscle, hair follicles, and brain. AR is a phosphoprotein that regulates MAP kinase activity, and MEK1/2 pathway inhibition correlates with changes in AR phosphorylation state. Abnormal AR signaling is linked to diseases including prostate cancer, Kennedy’s disease, and male infertility. Mutations in the AR gene are associated with complete androgen insensitivity (CAIS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.